Evaluation of Using Amylopectin of White Rice (Oryza sativa) Endosperm Powder in Substituting Agar for the Preparation of Growth Media for Culturing Microorganisms

Mona W Abdalla, Yassir Mohammed Abdelrahim and Mutaman AA Kehail*

Agar is usually mixed with other nutrients to make media, in which microorganisms can be cultured and tested. This study aimed to formulate white rice powder to substitute agar for the preparation of growth media to culture some bacterial and fungal strains. Following the standard methods, nutrient agar, potato dextrose agar, and blood agar media were carefully prepared. Following the same methods, rice-based media were prepared by adding rice powder instead of agar. Pure strains of E. coli, Staphylococcus aureus, Rhizopus stolonifer, and Aspergillus niger were used to evaluate growth rates (colony formation for bacteria) and mycelial radial growth (for fungi) within agar-based media and rice-based media. There was no significant difference between the growth rate within agar-based media and rice-based media for bacterial strains, but fungal strains were well flourished better within the rice-based media than bacterial strains. Although rice is a potential material for the solidification of nutrient media instead of agar, it can also add important nutritional components, and the amount of rice sufficient to result in the required solidification should be considered, which involves rice varieties.

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Published on: Nov 27, 2025 Pages: 9-13

Full Text PDF Full Text HTML DOI: 10.17352/ach.000038
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