Detection of the Mixed Disulfide Coenzyme A-S-S Glutathione: A Hypertensive Factor in Human Plasma of Young and Adult Individuals With or Without Hypertension

Raul N Ondarza V, Eva Hernandez M and Mauricio A Ondarza B*

Background: We have previously demonstrated the presence of a nucleotide peptide from rat liver which was identified as a mixed disulfide formed by Coenzyme A-SS-Glutathione linked by a disulfide bridge. Also informed about the presence in rat liver and other tissues of the NADPH-dependent Coenzyme A-SS-G reductase. Now we communicate about the presence of Coenzyme A-SS- Glutathione as a hypertensive agent in human plasma of 22 young and adult individuals with or without hypertension. 

Methods: In the present work the isolation of Coenzyme A-SS-Glutathione from rat liver to be used as standard and the human blood samples were extracted with Perchloric acid (HCLO4) neutralized with 2 N KOH; the supernatant was lyophilized and dissolved in a minimal amount of water in order to be separated by High Pressure Liquid Chromatography (HPLC) system with UV detector capable of measurement at 254 nm. 

Results: We have demonstrated for the first time the presence in human blood of young and adult individuals of the hypertensive compound CoASSG. Two subgroups could be observed among the young individuals according to the amount of CoASSG in plasma: in subgroup number 1 an average amount of CoASSG/ul of plasma of 1.895 and in group number 2 with an average amount of 7.92.

In the group of adults also two subgroups could be detected: subgroup 3 with an average of 2.756 and subgroup 4 with an average of 12.95. 

Conclusion: According to our results it can be appreciated that the mixed disulfide compound (CoASSG) is present in the blood of young individuals and in higher amounts in older people.

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Published on: Aug 22, 2025 Pages: 1-4

Full Text PDF Full Text HTML DOI: 10.17352/ach.000036
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