Development of a Method of Isolating Exosomes from Cell Cultivations

Desyatova M, Antonova S, Ufimtseva M, Korotkov A and Makeyev O*

The results of epidemiological studies conducted in divergent countries demonstrate the high prevalence of Atopic Dermatitis (AD) and the dynamics of the increase in the incidence of this pathology over the past decades. According to current research, AD is a multi-factorial disease, the pathogenesis of which is associated with both mutations in genes encoding epidermal structural proteins and epigenetic changes in gene expression. Generally accepted methods of treating skin damage in AD allow achieving remission, but not achieving a complete cure. It is shown that the composition of exosomes having a plasma origin can be supplemented with biologically active molecules introduced into the pericellular medium. The results obtained under the conditions of damage modeling in in vitro suggest that the use of exosomes for correcting the epigenetic landscape of cells under in vitro damage conditions is promising. 

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Published on: May 28, 2025 Pages: 8-12

Full Text PDF Full Text HTML DOI: 10.17352/2455-8605.000054
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